Class MuscleCommandline

source code

                     object --+    
                              |    
Application.AbstractCommandline --+
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                                 MuscleCommandline

Command line wrapper for the multiple alignment program MUSCLE.

http://www.drive5.com/muscle/

Example:

>>> from Bio.Align.Applications import MuscleCommandline
>>> muscle_exe = r"C:\Program Files\Aligments\muscle3.8.31_i86win32.exe"
>>> in_file = r"C:\My Documents\unaligned.fasta"
>>> out_file = r"C:\My Documents\aligned.fasta"
>>> muscle_cline = MuscleCommandline(muscle_exe, input=in_file, out=out_file)
>>> print muscle_cline
C:\Program Files\Aligments\muscle3.8.31_i86win32.exe -in "C:\My Documents\unaligned.fasta" -out "C:\My Documents\aligned.fasta"

You would typically run the command line with muscle_cline() or via the Python subprocess module, as described in the Biopython tutorial.

Citations:

Edgar, Robert C. (2004), MUSCLE: multiple sequence alignment with high accuracy and high throughput, Nucleic Acids Research 32(5), 1792-97.

Edgar, R.C. (2004) MUSCLE: a multiple sequence alignment method with reduced time and space complexity. BMC Bioinformatics 5(1): 113.

Last checked against version: 3.7, briefly against 3.8